APOBEC (apolipoprotein B mRNA-editing enzyme catalytic-polypeptide) family members were reported as innate immune molecules with anti-viral activity for many viruses, such as HIV and HBV. In order to understand the function of APOBEC, the APOBEC-3F and -3G were cloned, expressed, and the sub-cellular localization of them was detected. The genes of APBEC-3F and -3G were cloned from PHA-stimulated PMBC and expressed in the MDCK cell by transfection. The sub-cellular localization of APOBEC-3F and -3G were detected by immunofluorescence. APOBEC-3F and -3G were cloned by RT-PCR and confirmed by DNA sequencing. The immunofluorescence indicated APOBEC-3F and -3G were located in the cytosal. APOBEC-3F and -3G could inhibit HBV replication effectively in HepG2.2.15 cell. APOBEC-3F and -3G could not be trans-located into nuclear by nuclear location signal (NLS) or bi-NLS (B-NLS). These results will help the future research on the function of APOBEC.