To investigate function of collapsin response mediator protein-1(CRMP-1) on neurite outgrowth, the CRMP-1-phrGFPⅡ-N eukaryotic expression vector was constructed and transfected into PC12 cells induced by nerve growth factor. The lapse-time imaging and neurite extraction, as well as immunoblotting were utilized. The results demonstrated that PC12 cells were induced to be typical neurons by nerve growth factor. Lipofectamine could effectually transfect CRMP-1 into PC12 cells induced by nerve growth factor. Overexpression of CRMP-1 inhibited neurite outgrowth and collapsined the tiny neurite, then the longer neurite. Length of neurites demonstrated a tendency to be gradually shorten accompanying expression of CRMP-1 protein. Neurite extraction showed that cells overexpressing CRMP-1 possesed more and longer neurite(P < 0.01), compared with cells only induced by nerve growth factor and transfected by expression vector without CRMP-1. These data suggested that CRMP-1 play an important role in inhibiting neurite outgrowth.