To investigate transduction mechanism on carboxyl terminal activating region 3 of Epstein - Barr virus encoded latent membrane protein 1(LMP1) in nasopharyngeal epithelial cells NP69, the NP69 cell lines were respectively infected, applied the approach of retroviruses infection, by RV-LMP1 and RV-LMP1^Δ252～351 retroviruses. Therefore, the NP69-LMP1 and NP69-LMP1^Δ252～351 cell lines of stable expression LMP1 were established. Sequentially, cellular proliferation of the NP69-LMP1 and NP69-LMP1^Δ252～351 cell lines was compared to draw growth curve, experiment plate clone formation and test soft agar colony forming. Meanwhile, adopted proteomic methods, the differential expression proteins were identified between NP69-LMP1and NP69-LMP1^Δ252～351 cell lines, and the expression levels of partial identified proteins were verified by Western blotting and fluorescent real-time quantitative RT-PCR. The results showed: 1) the ability of LMP1^Δ252～351 promoting NP69 cell proliferation was obviously decreased to compare with wild type LMP1(n=3，P < 0.05). 2) 16 proteins, 8 up- and 8 down-regulated ones, of LMP1-CTAR₃ mediated regulation were identified from infected NP69 cell lines. 3) The differential expression of partial identified proteins was similar with 2-DE separated ones and confirmed by Western blotting and fluorescent real-time quantitative RT-PCR. These results suggested that LMP1-CTAR₃ is the important activating domain of inducing cellular proliferation and the domain probably plays the role of mediating to regulate the expression of G-protein, isocitric enzyme, and so on.