This work was supported by a grant from The National Natural Science Foundation of China (30571416)
Temporins 是从蛙属中得到的一类羧基端酰胺化的疏水性抗菌肽,具有抗细菌、霉菌、酵母菌、原虫及病毒活性.为了研究牛蛙皮肤抗菌肽的多样性及其结构特点,根据GenBank数据库中蛙属抗菌肽基因信号肽序列设计简并引物,从牛蛙皮肤cDNA文库中克隆到两个新的temporins 家族抗菌肽,命名为 temporin-La (LLRHVVKILEKYLamide) 和 temporin-Lb (LFRHVVKIFEKYLamide).合成的 temporin-La 和 temporin-Lb肽具有很强的抗菌活性,尤其是对革兰氏阳性细菌.溶血性测定结果表明,temporin-La 浓度高至250 mg/L 时对兔红细胞仍无溶血活性,而 temporin-Lb 具有较弱的溶血活性(半数致死浓度LC50 ≈ 230 μmol/L).通过透射电镜观察 temporin-La 和 temporin-Lb 处理过的金黄色葡萄球菌的细胞结构,发现它们都能直接地杀死细菌,但作用机制不一样.
Temporins are a kind of small, hydrophobic and C-terminus amidated antimicrobial peptides from Rana species. They are effective against bacteria, fungi, yeast, protozoa and viruses. Two novel temporins named as temporin-La(LLRHVVKILEKYLamide) and temporin-Lb(LFRHVVKIFEKYLamide) were cloned from Lithobates catesbeianus. Synthetic peptides of temporin-La and temporin-Lb showed strong antimicrobial activities against bacteria tested, especially Gram-positive bacteria. Besides, temporin-La showed no haemolytic activity to rabbit erythrocytes at the concentration of 250 mg/L while temporin-Lb showed weak haemolytic activity(LC50 ≈ 230 μmol/L). Transmission electron microscopy showed that temporin-La and temporin-Lb induced different effects on bacterial structure of Staphylococcus aureus.
赵瑞利,韩俊友,韩文瑜,雷连成,孙长江,冯 新,江丽娜,乔红伟,蔡林君.牛蛙两个新Temporins基因的克隆及其抗菌机制的研究[J].生物化学与生物物理进展,2009,36(8):1064-1070
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