Mammary stem cells (MaSCs) is an ideal model for studies of organogenesis, cell proliferation, differentiation, survival and apoptosis. Recent researches showed that many adult stem cell surface markers belonged to cell adhesion molecule (CAM) family. Therefore, analysis of relationship between mammary stem/progenitor cells and the expression pattern of CAM have directive meanings for identifying embryonic mammary stem/progenitor cells and understanding their properties. Here, cells in E14 mouse mammary anlagen were purified using the adult mouse mammary epithelial stem cell (MaESC) markers of CD24 and CD49f. It was found that CD24 and CD49f double-positive cells contain two distinct cell populations: CD24^hiCD49f⁺ and CD24^medCD49f⁺, and their percentages in total mammary anlagen cells were 16% and 47%, respectively. In the following monolayer culture and in vivo transplantation tests, it was found that the CD24^medCD49f⁺ cells could attach plate and regenerate mammary ductal units; correspondingly, CD24^hiCD49f⁺ cells did not possess of these capabilities. These results indicate that the two mammary anlagen cell populations represent different cell types, and CD24^medCD49f⁺ population may contain the self-renewal mammary anlagen stem/progenitor cells. Afterwards, the differences in the expressions of 19 mammary-related CAM transcripts in the two cell populations were validated by quantitative real-time PCR analysis. The data show that the CAM gene expressions of mammary repopulating-CD24^medCD49f^{+ cells are dramatically different from that of CD24hiCD49f+ cell population. The expressions of several adult stem cell markers in CD24medCD49f+ cell population are remarkable higher than those in CD24hiCD49f+ cell population.}