RING1 can bind to DNA and inhibit some gene transcription. RING1 was identified to interact with lamin A by screening a human skeletal muscle library in a yeast two-hybrid interacting screen. The results of one to one reverse hybridization indicated that AH109 transfected with RING1 and lamin A constructs could grow on SD/-Leu/-Trp/-Ade/-His medium. RING1 was inserted into pEGFP-N1 vector and the constructs including pDsRed-LA and pEGFP-RING1 was used to transfect HEK293 cells. Visualized by laser confocal microscopy, RING1 and Lamin A were co-located around nucleus. Interatcion of RING1 and lamin A was comfirmed by immunoprecipitations. These results showed a new lamin A binding protein that can present some evidence for lamin A acting on gene expression and cell senescence.