蛋白质结构与相互作用研究新方法——交联质谱技术

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蛋白质结构与相互作用研究新方法——交联质谱技术
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作者单位:中国科学院计算技术研究所智能信息处理重点实验室,中国科学院计算技术研究所智能信息处理重点实验室,北京生命科学研究所,中国科学院计算技术研究所智能信息处理重点实验室,中国科学院计算技术研究所智能信息处理重点实验室,北京生命科学研究所,中国科学院计算技术研究所智能信息处理重点实验室,中国科学院计算技术研究所智能信息处理重点实验室,中国科学院计算技术研究所智能信息处理重点实验室,北京生命科学研究所,中国科学院计算技术研究所智能信息处理重点实验室
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基金项目:国家自然科学基金面上项目(21475141)和国家重点基础研究发展计划(973)资助项目(2010CB912701, 2012CB910602, 2013CB911203)

A New Approach to Protein Structure and Interaction Research: Chemical Cross-linking in Combination With Mass Spectrometry

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Key Laboratory of Intelligent Information Processing,Institute of Computing Technology,The Chinese Academy of Sciences,Key Laboratory of Intelligent Information Processing,Institute of Computing Technology,The Chinese Academy of Sciences,National Institute of Biological Sciences,Key Laboratory of Intelligent Information Processing,Institute of Computing Technology,The Chinese Academy of Sciences,Key Laboratory of Intelligent Information Processing,Institute of Computing Technology,The Chinese Academy of Sciences,National Institute of Biological Sciences,Key Laboratory of Intelligent Information Processing,Institute of Computing Technology,The Chinese Academy of Sciences,Key Laboratory of Intelligent Information Processing,Institute of Computing Technology,The Chinese Academy of Sciences,Key Laboratory of Intelligent Information Processing,Institute of Computing Technology,The Chinese Academy of Sciences,National Institute of Biological Sciences,Key Laboratory of Intelligent Information Processing,Institute of Computing Technology,The Chinese Academy of Sciences

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This work was supported by grants from The National Natural Science Foundation of China(21475141), and The National Basic Research Program of China (2010CB912701, 2012CB910602, 2013CB911203)

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摘要:

蛋白质的空间结构信息以及蛋白质间的相互作用信息对于研究蛋白质的功能有重要意义．研究蛋白质结构与相互作用的传统技术，如核磁共振技术、X射线晶体衍射技术等，对于蛋白质的纯度、结晶性和绝对量均有比较高的要求，限制了其广泛应用．交联质谱技术是近十多年来发展起来的新技术，它将质谱技术与交联技术相结合，在研究蛋白质结构与相互作用方面具有速度快、成本小、蛋白质各方面性状要求低等优势．本文就交联质谱技术各个环节的技术方法加以综述，包括交联质谱实验分离富集技术、常见交联剂特性、交联质谱数据库搜索算法、结果验证研究和交联质谱技术的应用等方面，并展望了该研究方向未来的发展．

Abstract:

For any protein, its 3-D structure and interaction with other proteins are vital information for the understanding of its function. Traditionally, NMR and X-ray crystallography are employed to acquire such information at high resolution. However, application of these methods is limited because they are extremely demanding for protein purity and quantity, or protein size and crystalizability. Chemical cross-linking in combination with mass spectrometry (CXMS) is a novel technology developed over the past decade that complements the traditional methods with its many advantages such as high throughput, low cost and relaxed sample requirements. Here we review the various aspects of CXMS, including separation and enrichment techniques, characteristics of common cross-linkers, development of database-search tools for CXMS data, algorithms for validation of results, and application. The future development of CXMS is also discussed.

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樊盛博,吴妍洁,杨兵,迟浩,孟佳明,卢珊,张昆,邬龙,孙瑞祥,董梦秋,贺思敏.蛋白质结构与相互作用研究新方法——交联质谱技术[J].生物化学与生物物理进展,2014,41(11):1109-1125

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收稿日期:2013-11-29
最后修改日期:2014-04-18
接受日期:2014-05-26
在线发布日期: 2014-11-21
出版日期: 2014-11-20

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