1
蓝藻生物钟核心振荡器的KaiB-KaiC相互作用机制
The Mechanism of KaiB-KaiC Interaction of The Cyanobacterial Circadian Oscillator
投稿时间:2014-07-20  修订日期:2014-11-28
中文关键词:  生物振荡器,生物钟,KaiB,KaiC
英文关键词:circadian oscillator, circadian clock, KaiB, KaiC
基金项目:国家自然科学基金(21103098),北京分子科学国家实验室开放基金和三峡大学科研基金(2011071001,KJ2012B004,KJ2014H015)资助项目
作者单位E-mail
刘松 三峡大学医学院分子生物学研究所宜昌 443002 songliu_biox@163.com 
刘森 三峡大学医学院分子生物学研究所宜昌 443002 senliu.ctgu@gmail.com 
摘要点击次数: 1329
全文下载次数: 2065
中文摘要:
      蓝藻是已知的具有昼夜节律生物钟调控机制的最简单生物,其生物钟的核心是一个由三个蛋白质(KaiA、KaiB、KaiC)组成的,不依赖于转录翻译水平调控的核心振荡器.研究表明这三个蛋白质仅在体外试管中反应就会表现出周期性磷酸化振荡现象.分子水平研究表明:KaiA加速KaiC的自磷酸化,而KaiB抑制KaiA使KaiC去磷酸化,从而KaiC的磷酸化/去磷酸化形成周期性反复.但是KaiB如何与KaiA,KaiC相互作用,目前还不清楚.本文重点介绍了最近几年来在KaiB-KaiC相互作用机制上的研究进展,并结合我们的一些初步研究,对KaiB-KaiC相互作用的关键问题进行展望,以期为该体系的深入研究提供参考.
英文摘要:
      Cyanobacteria are the simplest organisms with a confirmed circadian clock system. The pacemaker of cyanobacterial circadian clock is made of three proteins, KaiA, KaiB, and KaiC. A major finding on this system is that the circadian oscillation of the pacemaker system is independent of transcriptional/translational controls, and what is more intriguing is that this oscillation can be reconstituted in vitro with only these three proteins, in addition to ATP and an appropriate inorganic buffer. Molecular studies have shown that KaiA promotes the self-phosphorylation of KaiC, whereas KaiB antagonize KaiA's role and induce the de-phosphorylation of KaiC. An unsolved question is how KaiB interacts with KaiC exactly, including the binding site of KaiB on KaiC, the oligomerization form of KaiB, and the exact modulating mechanism of KaiB. Here, we reviewed the most recent progress on the KaiB-KaiC interaction mechanism, including our preliminary results on the KaiB-KaiC interaction, and provided a possible molecular mechanism of the molecular oscillator. We hope this review will provide a timely perspective on the study of this model system.
HTML  查看全文  查看/发表评论  下载PDF阅读器
  
京期出证字第0624号 版权所有:  您是本站第    位访问者
主管单位:中国科学院 主办单位:中国科学院生物物理研究所和中国生物物理学会 地址:北京市朝阳区大屯路15号
电话:010-64888459 电子邮件:prog@sun5.ibp.ac.cn
技术支持:北京勤云科技发展有限公司
京ICP备05023138号-1

京公网安备 11010502031771号