After optimizing the conditions of surface modification, sieving matrix, electric field strength of separation, injection mode, electrophoresis temperature, injection time and so on, forensic DNA samples included 15 STR loci can be separated on Brofloat glass electrophoresis chip, using electrophoresis apparatus equipped with confocal laser induced fluorescence detection system. The optimized electrophoresis system conditions were obtained and DNA samples were successfully separated within 8min. The microchip electrophoresis system has good prospects in the rapidly forensic DNA analysis.