Uveitis is a recurrent inflammatory disease that can lead to immune system dysfunction and multiple organ injuries. However, whether uveitis causes liver functional damage is still unclear. Herein, by using flow cytometry and confocal, we investigated the pathological and functional changes of liver in an experimental autoimmune model of uveitis (EAU). Hepatic damage was observed at the inflammatory stage of uveitis and was associated with severity of eye damage. Moreover, the expression of CD3⁺CD4⁺ T cells, CD3^-NK1.1⁺DX5^- NK cells, and CD11b⁺F4/80^-Ly6c⁺ cells were increased in the inflamed eye and liver. Furthermore, the pathological damage of EAU and hepatic impairment were aggravated after transferring CD3⁺CD4⁺ T cells into EAU mice. Additionally, vascular dilation and infiltration of CD3⁺CD4⁺ T cells were found in the eyes and livers of EAU mice. In conclusion, our findings suggest that liver injury could occur in EAU. This liver injury may be associated with increased CD3⁺CD4⁺ T cells, which may infiltrate into liver through circulatory system.