综述与专论: 纳米酶在分析化学中的应用研究:从体外检测到活体分析
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南京大学 现代工程与应用科学学院,南京大学 现代工程与应用科学学院,南京大学 现代工程与应用科学学院,State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun,南京大学 现代工程与应用科学学院

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国家自然科学基金(21722503, 21405081), 国家重点基础研究发展计划(2015CB659400), 江苏高校优势学科建设工程、江苏省双创计划、生命分析化学国家重点实验室开放基金(SKLACLS1704)和国家千人计划青年项目资助


Reviews and Monographs: Nanozymes in Analytical Chemistry: From in vitro Detection to Live Bioassays
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Department of Biomedical Engineering,College of Engineering and Applied Sciences,Collaborative Innovation Center of Chemistry for Life Sciences,Nanjing National Laboratory of Microstructures,Nanjing University,Nanjing,Department of Biomedical Engineering,College of Engineering and Applied Sciences,Collaborative Innovation Center of Chemistry for Life Sciences,Nanjing National Laboratory of Microstructures,Nanjing University,Nanjing,Department of Biomedical Engineering,College of Engineering and Applied Sciences,Collaborative Innovation Center of Chemistry for Life Sciences,Nanjing National Laboratory of Microstructures,Nanjing University,Nanjing,State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun,Department of Biomedical Engineering,College of Engineering and Applied Sciences,Collaborative Innovation Center of Chemistry for Life Sciences,Nanjing National Laboratory of Microstructures,Nanjing University,Nanjing

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This work was supported by grants from The National Natural Science Foundation of China (21722503, 21405081), National Basic Research Program of China (2015CB659400), PAPD Program, Shuangchuang Program of Jiangsu Province, Open Funds of the State Key Laboratory of Analytical Chemistry for Life Science (SKLACLS1704), and Thousand Talents Program for Young Researchers

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    摘要:

    纳米酶是指具有类酶催化活性的纳米材料.近年来,纳米酶研究引起了人们的极大兴趣.纳米酶已被广泛应用于诸如生物传感、生物成像、疾病治疗和环境保护等众多领域.在本综述中,我们将着重讨论纳米酶在分析化学领域的研究进展.首先将讨论纳米酶在体外检测的应用,将包括生物活性小分子、核酸、蛋白质类生物标志物、细胞等的检测.其后将讨论纳米酶在活体分析的应用,将包括监测活脑、肿瘤组织等的生物活性小分子、药物的药效、药物与纳米酶的代谢等.最后,我们将讨论纳米酶应用于分析化学时面临的挑战和未来研究前景.

    Abstract:

    Nanozymes, the catalytic nanomaterials with enzyme-like properties, have attracted enormous interests in recent years. They have been used for wide range applications from biosensing and bioimaging to therapeutics and environmental protection. In this review, we highlighted the recent progress of nanozymes in analytical applications. We first discussed the in vitro applications, which covered the detection of bioactive small molecules, nucleic acids, protein biomarkers, cells, etc. We then discussed the in vivo applications, which included the monitoring of bioactive small molecules in live brains and tumor tissues, the study of drug efficacy, and the investigation of drug and nanozyme metabolism, etc. Finally, we concluded the potential challenges of nanozymes when applying to analytical chemistry and prospected future directions.

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李思蓉,黄彦钧,刘嘉睿,汪尔康,魏辉.综述与专论: 纳米酶在分析化学中的应用研究:从体外检测到活体分析[J].生物化学与生物物理进展,2018,45(2):129-147

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历史
  • 收稿日期:2017-12-18
  • 最后修改日期:2018-01-17
  • 接受日期:2018-01-19
  • 在线发布日期: 2018-02-05
  • 出版日期: 2018-02-20