Recent studies have revealed that DJ-1 is overexpressed in multiple forms of human tumors, and nuclear overexpression of DJ-1 is correlated with the biological behavior of tumor. The purpose of the present study was to test for possible DADS effects on Human Leukemia HL-60 cells which DJ-1 is overexpressed, and to clarify the function of DJ-1 in the nucleus. Gene transfection technology was used to establish stable HL-60 cell lines with nuclear overexpression of DJ-1 (DJ-1/HL-60). Soft agar colony formation, MTT, NBT and indirect immunofluorescence were used to evaluate the proliferation, differentiation, migration and invasion of HL-60 cells. The nuclear overexpression of DJ-1 protein may promote the proliferation and weaken the differentiation of HL-60 cells. Transwell migration and invasion assays showed that the nuclear localization and overexpression of DJ-1 can promote the migration and invasion capability of HL-60. Western blot analysis revealed that the expression of DJ-1 was inhibited in HL-60 cells with nuclear overexpression of DJ-1 after treatment with DADS. These results defined the impacts of DJ-1 on biological behavior of the Human Leukemia HL-60 cells by DADS, the proliferation, migration and invasion were inhibited and the differentiation was induced in HL-60 cells with nuclear overexpression of DJ-1, after treatment with DADS.