The centrosome is the principal microtubule organizing center in most animal cells. It ensures orderly cell cycle progression with accurate chromosome segregation. We have previously reported that the centrosomal protein Centlein functions as a molecular linker between C-Nap1 and Cep68 to maintain centrosome cohesion. To explore novel function of Centlein, in this study, we generated Centlein knockout cell lines, and performed RNA-seq and data analysis on Centlein knock out and control cells, in parallel. Ablation of Centlein upregulated PLK1, CCNB1, CCNA2 and CDC20, and promoted cell cycle progression. PLK1 protein, found elevated in Centlein knock out cells, interacted with Centlein in vivo. We propose that centrosomal PLK1 exerting control over the cell cycle relies upon the interaction with Centlein.