Reperfusion after acute myocardial infarction is the only way to rescue ischemic myocardium, but recovery of blood flow may lead to ischemia/reperfusion (I/R) injury. Long non-coding RNA (lncRNA) and pyroptosis are involved in the pathological process of myocardial I/R injury and play important roles in it. LncRNA can directly or indirectly act on pyroptosis signaling pathway related proteins, and then regulate various pathological processes including myocardial I/R injury. In this review the roles of lncRNA and pyroptosis in myocardial I/R are summarized to further explore the relationship between them and provide new ideas for the prevention and treatment of myocardial I/R injury.