Recent studies of cell biophysics have been introduced about cell ultrastructure effects of physical factors on living cells, cellular motilities, ion channels and cellular signalling. Some progress in methodology for studying living cells are also reviewed. It has been emphasized that the main purpose of cell biophysics is to understand the nature of the living cell in order to explain why and how the cell is alive. Accordingly, some questions and problems on principles and methodology have been discussed in this article as well.