Vacuum ultraviolet circular dichroism (VUV-CD) spectra of proteins in solution have been measured using synchrotron radiation vacuum ultraviolet circular dichroism spectrometer and a special cell. The measurement wavelength is down to 175nm. A new calculation method has been applied for calculating the content of five kinds of secondary structures of proteins. Their results are coincident with that from X-ray diffraction method. In order to get good VUV-CD spectra, several important factors have been discussed. The experiments show that so far. VUV-CD analysis is one of the favorable method for secondary structure studies of proteins.
Lin Bo-hai. Vacuum Ultraviolet Clrcular Dichroism Studies on Secondary Structure of Proteins[J]. Progress in Biochemistry and Biophysics,1994,21(1):67-69
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