Sephadex LH-20 and silica gel centrifugal liquid chromatography were applied to isolation and purification of gangliosides from the pig brain. The highly purified gangliosides were obtained. The concentration of lipid-bound sialic acid determined is 30.1% (W/W). The results determined by silica gel G-60 HPTLC and 580nm scanning were GM1 19.5%. GD3 13.8%, GD1a 27.8%, GD1b 14.2% and GT1b 19.3%.