The aqueous solution of the silk fibroin from silkworm Bombyx mori cocoons was obtained by treatment of the neutral salt (CaCl2). Peroxidase(POD)was immobilized in the silk fibroin membrane. Activity and characteristics of the POD-immobilized fibroin membrane were analysed.The results show that the activity of the POD-immobilized membrane is high;Optimum pH range for the enzyme membrane,being at pH5.0-7.0,was widened in comparison with the free enzyme;and its thermal stability was good.
Zhang Yuqing, Zhou Yuzhen. Preperation of the Peroxidase-Immobilized Fibroin Membrane and Its Characteristics[J]. Progress in Biochemistry and Biophysics,1995,22(2):162-165
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