According to the rules of eukaryotic gene expression and regulation,two versatile eukaroytic expression vectors(pMAML1-dhfr and pMAML2-dhfr) originated from pSV2-dhfr were constructed.They both have a CMV immediate early promoter/enhancer and consist of two expression units of parallel or opposite orientation.Using firefly luciferase gene as reporter gene and β-galactosidase gene as internal control,their effects on luciferase expression were studied in COS-7 transient expression system.The relative strength of their cis-elements were compared with those in pSV2-dhfr.
Long Jianyin, Zhang Hongquan, Wang Huixin. Construction of Versatile Eukaryotic Plasmid Expression Vectors[J]. Progress in Biochemistry and Biophysics,1995,22(4):330-334
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