Genomic subtraction is an efficient and economical method for isolating sequences pressent in one genomic DNA population(tester)but absent in another(driver).It proved widely applicable to detecting genomic abnormalities in cancer,probes for polymorphic loci and acquisition of new sequences in infectious diseases of viral organism. The fundation and development of genomic subtraction,experimental strategy,applications, problems in present and promise in future are described.
Hou Ping, Wu Min. The Method and Applications of Genomic Subtraction[J]. Progress in Biochemistry and Biophysics,1995,22(5):394-397
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