A microsome preparation procedure using polyethylene glycol(PEG )is reported. The liver homogenate of rats is aggregated by polyethylene glycol-6000,and centrifuged at high speed two times.The obtained pellet is the microsomal fraction. Compared with the ultracentrifugation method,this procedure of using PEG to prepare microsomes greatly eliminated the need for an ultracentrifuge and reduces the time necessary for isolation of this fraction. It is simple and feasible.
Wang Lie, Yang Mingxue, Xie Guangyun. Preparation of Microsomes with Polyethylene Glycol[J]. Progress in Biochemistry and Biophysics,1995,22(5):462-464
Copy® 2024 All Rights Reserved ICP:京ICP备05023138号-1 京公网安备 11010502031771号