Laboratory investigations for one step purification of recombinant human IL-4 (rhIL-4)with cation ion-exchange chromatography are presented.The method is easy to operate and gives no dilution effect on the sample.The purified rhIL-4 is of high purity (≥95%),high biological activity(≥10 MU/mg) and low toxicity(ET≤0.5 ZU/L),which meets the criteria of cytokines for the further experiemental research.Furthermore,purity of rhIL-4 can be increased as high as 98% with higher biological activity when two steps of purification were employed by using combination of ion exchange and gel-filtration chromatographies.
Wang Hong, Wang Jinjuan, Li Yan, Liu Jie, Chen Weifeng. Purification of Recombinant Human Inter-leukin-4 with Ion-Exchange Chromatography[J]. Progress in Biochemistry and Biophysics,1995,22(5):468-472
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