The mammalian zona pellucida plays a major role in the fertilization. The study of chemical substances in zona Pellucida and its bioactivities is of importance in studying the molecular mechanism of species-specific spermegg recognition and the induction of acrosome reaction. Studies in latest ten years show that zona pellucida mainly consists of glycoproteins which are synthesized inside oocyte and then tranferred to the outside of oocyte. Most animals have 4 or 5 kinds of ZPGPs, and of which proteins possess homologous. During the process of fertilization, ZPGPs are responsible for spermatozoa receptors and for the induction of acrosome reaction. ZPGPs contain N-linked and O-linked oligosaccharides, which are essential to the bioactivities of ZPGPs.