Random or arbitrary primers, in contrast with common specific primer,refer to the non-specific oligonucleotides used as primers in DNA synthesis.In 1990's several new techniques were developed as a result of combination of random primers and PCR. RAPD, AP-PCR and DAF by random primers with different length were used for DNA fingerprinting. Differential display was used for polymorphism analysis of mRNA.rPCR,T-PCR and so on derived from application of random primers were discussed too.The technique characters and applications in molecular biology research of random primered PCR were introduced particularly in RAPD.
Liu Chunyu, Zhang Chuming, Xia Jiahui. Application of Random Primers in the Research of Molecular Biology[J]. Progress in Biochemistry and Biophysics,1996,23(6):517-520
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