Ganglioside GM3 and bovine brain Gangliosides(BBG) were added exogenously to human glioblastma multiform cell line BT325 to observe their effects on BT325. The results showed that GM3 and BBG inhibited BT325 cell growth. The maximum of cell proliferation inhibition rate of GM3 and BBG were 60.28% and 19.33%, respectively. GM3 and BBG were added to medium which has different concentration of EGF. Both GM3 and BBG inhibited the EGF-stimulated BT325 cell growth and the effects of GM3 is far stronger than BBG.