The methylotrophic yeast, Pichia pastoris heterologous gene expression system was utilized to produce attractive levels of a variety of intracellular and extracellular proteins of interest. Lateolabrox japonicus growth hormone gene was cloned into the yeast integrative vector pHIL-D2, which was then transformed into his4 mutant yeast GS115. PCR fast detection methods was performed to screen the positive transformants and dot-blotting was used to screen the multiple-copy transformants. Via inducing AOX1 promoter by methanol, growth hormone could be expressed intracellularly. SDS-PAGE and Western blot were applied to confirm the product.
CHEN Dan, YANG Feng, WANG Wei, XU Xun. Intracellular Expression of Lateolabrox japonicus Growth Hormone in Methyltrophic Yeast, Pichia pastoris[J]. Progress in Biochemistry and Biophysics,1998,25(2):140-143
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