The triplex formation between a 21nt oligodeoxyribonucleotide G3TG2TGT2G5TG2TGT(CP1) and core promoter(Cp) fragment of hepatitis B virus(HBV) had a good specificity and stability, which was demonstrated by electrophoretic mobility shift analysis and DNaseⅠ footprinting experiment. Gel retardation assay showed CP1 could inhibit a specific cellular factor binding to Cp fragment in rat liver nuclear extracts. No inhibition of factor binding was observed by oligodeoxyribonucleotide CP3(TGTG2TG5T2GTG2TG3), which could not form triplex with Cp fragment. These results indicate that specific repression of gene transcription of HBV DNA may be possible by triplex-formation.
LIU Ding-xie, WANG Chang-cai. Inhibition of DNA Binding Protein Binding to the Core Promoter of Hepatitis B Virus by Triplex-Formation[J]. Progress in Biochemistry and Biophysics,1998,25(3):262-266
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