Hepatitis C virus(HCV) is a major causative agent of non-A non-B chronic hepatitis and also associated with the development of hepatocellular carcinoma. To understand the mechanism of pathogenesis and tumorigenesis of HCV and the role of the structural protein and NS3 protein in the HCV infection,three lines of transgenic mice carrying the HCV structural gene or/and NS3 gene, in which these genes are expressed under the control of the mouse metallothionein promoter, were produced simultaneously. These animals were produced by microinjecting into the pronuclei of fertilized mouse eggs with the mixture of construct carrying the structural gene and NS3 gene of HCV. The results were as follow: 1) The zygote's survival rate and birth rate were 81% and 30%, respectively; 2) After screening 60 mice that developed from the microinjected eggs by PCR, 6(10%)mice carrying the structural gene, 4(6.7%)mice carrying NS3 gene and 9(15%) carrying double genes.The total integrate rate was 31.7%; 3)The expression of transgene in the liver of Tg mice was analyzed by detection of the mRNA using RT-PCR; 4) 38 G1 mice were obtained by crossbred founder mice and normal mice, 20 mice were integrated(52.6%) with transgene; 5) No phenotypic abnormalities have been seen to date. The model may create the opportunity to examine the role of the structural and NS3 protein in HCV pathogenesis and tumorigenesis, and to assess the antiviral potential of the pharmacological agents and vaccine.