Hepatitis C virus has four chief genotypes HCVⅠ、Ⅱ、Ⅲ and Ⅳ,whose homology of amino acids and nucliec acids is less than 80% each other.The variation rates of HCV proteins are core 8%，E1 35%，E2/NS1 53%，NS3 27%，NS4 35%，NS5 39%，respectively. According to the characters of conservation, hydrophilicity, antigenicity and conformation,a series of highly conserved T cell and B cell epitopes are identified from the HCV expressed products,which might be benificial to the prevention and treatment of hepatitis C.In genenal, B epitope is about 12～40 peptides and T epitope 7～9 peptides.