Combined protein homologous modeling with analysis of electrostatic surface potential energy, mutant ricin toxin A chain, which was suggested to be bioactive, was designed. The gene of ricin toxin A chain was amplified by PCR and inserted in pKK223-3 to construct a prokaryotic expression vector, which was highly expressed in E.coli system. Moreover, the expression products showed obvious cytotoxicity, just in agreement with the prediction of modeling.
PEI Wu-Hong, FENG Jian-Nan, LEI Hong-Xing, LI Yan, SHEN Bei-Fen. The Modeling, Expression and Activity Study of Mutant Ricin Toxin A Chain[J]. Progress in Biochemistry and Biophysics,1999,26(3):270-272
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