In order to study the molecular pathology of hereditary persistence of fetal hemoglobin (HPFH) in Chinese, PCR-based chemical cleavage of mismatch (CCM) method was used for defining the point mutations causing non-deletional HPFH. 415 bp ^Gγ- and ^Aγ- globin gene promoter regions of 4 previously identified mutants were amplified by nested PCR, then the mutations were detected by CCM, and the optimal condition was determined. It was proved that CCM is a simple and reliable molecular diagnostic method for the detection of nd HPFH point mutations.