Androgen Insensitivity syndrome (AIS) is a X-linked recessive disease mainly associated with the defect of androgen receptor(AR). A method was established by which the AR gene can be amplified by PCR directly from the dried blood spot or from the wash-fluid of dried blood spot. The PCR products from dried blood spot have good specificity. Combining this method with SSCP and direct DNA cycle sequencing，the point mutations and the fragment changes of AR gene can be identified.This method is simple, economical and it offers the possibility of mutation analysis of AR gene or other genes from patients who live in remote countryside.