Total RNA was prepared from fat body of the silkworm, Bombyx mori, 9 hours after injected by E.coli K12D31. Single-strand cDNA was synthesized by reverse transcription (RT). Partial fragments of cecropin A cDNA were obtained, cloned and sequenced, by PCR technique with a pair of degenerate primers designed according to the amino acid sequence of CM4 in Bombyx mori and cecropin A in Hyalophora. These laid a foundation for further research on preparation of the probes of cecropin A for screening the silkworm cDNA library.
LI Jian-Min, ZHOU Kai-Ya, ZHANG Shuang-Quan, DAI Zhu-Ying, ZHU Chang-Liang, YE Xin-Hai. Sequencing Partial Fragment of Cecropin A cDNA in the Silkworm Bombyx mori From China[J]. Progress in Biochemistry and Biophysics,1999,26(4):399-402
Copy® 2024 All Rights Reserved ICP:京ICP备05023138号-1 京公网安备 11010502031771号