Trypsin remains active after autoclaving at 120℃ in acidic solution. This method of sterilization has been used routinely in cell culture work and found more simple, reliable and economical.
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HE Yuan-Zheng, LIU Ding-Gan. Sterilization of Trypsin by Autoclaving[J]. Progress in Biochemistry and Biophysics,1999,26(4):403-404
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