A eukaryotic vector highly expressing PKC_γ,was constructed by DNA recombination and transfected into C₃H₁₀T_1/2 cells by gene transfection. The NCP4 cells stably expressing PKC_γ was isolated successfully by Southern blot,Western blot and PKC activity assay.The NCP4 cells displayed an enhanced growth rate,especially under low serum conditions, apparently reduced dependence on serum. By using FCM, it was shown that NCP4 cells exhibited decreased percentage in G1 phase, increased percentage in S phase and G2+M phase. In contrast to control cells, NCP4 cells overexpressing PKC_γ decreased anchorage dependence and formed small colonies in soft agar. Furthermore, it was observed that the expression of oncogene c-sis increased obviously in NCP4 cells, it may be one of the molecular mechanisms of the reduced dependence on serum in NCP4 cells. The results indicated that specific elevation of the PKC_γ level directly affected the increase of growth rate and lead to some transforming phenotypes in C₃H₁₀T_1/2 cells.