A nested PCR was used to amplify the rare target sequence and 5% formamide was used to eliminate the nonspecific amplification. These modifications make clone the flanking sequence adjacent to the known sequence of human genome very effective and highly specific.
LI Zhu-Hong, LIU De-Pei, LIANG Chih-Chuan. A Modified Inverse PCR Method for Cloning the Flanking Sequences Adjacent to the Known Sequence in Human Genome[J]. Progress in Biochemistry and Biophysics,1999,26(6):600-602
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