A fast staining-destaining method for SDS-PAGE which favours the recovery of proteins was developed:brief staining by Coomassie Brillant Blue R-250 followed by destaining with 250 mmol/L KCl.The SDS-PAGE stained-destained by this method has almost the same clearance compared with the one stained-destained by the usual method,and the recovery of protein can be enhanced more than three times by this method.