PCR products amplified with Taq and Taq+Pwo DNA polymerase were reamplified by using UT-PCR and then cloned and sequenced. The termini of the original PCR products were analyzed. In the group of Taq amplification, the termini were chiefly sticky ones with 3′-protruding A(67.3%);the next majority of termini were blunt ones(26.9%).In the other group, the proportion of blunt-ends was almost the same as that in the group of Taq amplification(26.1%). However, the 3′-protruding termini decreased(17.4%),instead of additional 3′-recessive ones(-1,-2,-3;57.0%). The results reveal that the ends of PCR products are complicated.
XIA Qing-Jie, ZHANG Si-Zhong, WU Hui, XIAO Cui-Ying. Studies on Termini of PCR Products[J]. Progress in Biochemistry and Biophysics,2000,27(2):215-217
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