The high effective antibody of interleukin-6 was obtained by immunizing rabbits and guinea pigs with recombinant IL-6 many times. The IL-6 was labeled by ¹²⁵I with chloramines-T methods and purified by the Sephadex-G25 chromatograph column. The reaction between antigen and antibody was carried out by one step balance method and incubated in 4℃ for 24 hours, then separated bond and free antigen by PR reagent. The detection range of this method was about 0.1～3.2 μg/L, the lowest detection level was 0.1 μg/L, error within batches and between batches was less than 6.4% and 10% respectively. The serum IL-6 concentration in normal male was (0.270±0.13) μg/L (n=115), and in female was (0.260±0.10) μg/L (n=101), there was no difference in male and female group. Otherwise, the level of IL-6 in serum of rabbits was significantly higher than that of self-control at 24 hours after hemorrhagic shock and reperfusion. IL-6 in lymph fluid of rat after hemorrhagic shock was significantly elevated, then it was descended by the treatment of anisodamime (1 mg/kg). The liberation of IL-6 also was promoted and obviously higher than that of the control when fibre cells around tooth were cultured with endotoxin (10 mg/L) at different time in vitro.