The culture of callus and cell suspension induced from leaves of Rhodiola algida Var.tangutica collected from Qinghai plateau(altitude:4 000 m),on solid and in liquid culture medium(MS+BA₂+NAA_0.2)respectivelly,was successful.After cold (4～5℃)acclimation or ABA treatment for 10 or 14 days, the freezing resistance of suspension cells was increased. Proteins from the liquid suspension cell culture medium exhibited antifreeze activity. Treatment of the antifreeze proteins(AFPs) with boiling or trypsin caused inactivation,proving the protein characteristics of AFPs.Periodate and borate inhibited the antifreeze activity of AFPs,suggesting the presence of carbohydrate. Of the four treatments(25℃;25℃+ABA;5℃;5℃+ABA),2～3 polypeptides(29～50 ku) could be detected from the one dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis（SDS-PAGE）. PAS(periodic acid-schift’s reagent) reaction of SDS-PAGE showed the presence of carbohydrate in the polypeptides.