The RRTR can linearly increase cDNA products via repeated reverse transcription reactions controlled by temperatures and catalyzed by FD-TRT (FD thermostable reverse transcriptase). The stability of RNA in RRTR is proved via the Northern blot, and the increase in cDNA products along with that in reacting cycles is shown via the Dot hybridization. After components of the reaction system are optimized, the RRTR combined with PCR is applied in quantification of HCV RNA through changing the reacting cycles of the RRTR. It can be concluded that due to the linearity of RRTR, the RRTR is a convenient method to enhance the sensitivity and efficacy of RNA related study. It can be used in some fields which full-length cDNA is not required, such as quantification of RNA or cDNA, RACE （rapid amplification of cDNA end), and different display.