To understand the control of growth and glucose repression in Saccharomyces cerevisiae by glucose transport, a set of S.cerevisiae strains with variable expression of only one glucose transporter, Hxt7, the most abundantly expressed high-affinity transporter, was constructed. The strains were constructed by partial deletion of the HXT7 promoter in vitro and integration of the gene at various copy numbers into the genome of an hxtΔ (hxt1-hxt7 gal2 deletion) strain. The 149 bp DNA region －495 to －346 in the HXT7 promoter plays an important role in HXT7 expression. In the mutant strains with promoter length of more than 495 bp, the expression of HXT7 at high glucose concentrations was much higher than that in the wildtype strain. The level was dependent on copy number and promoter length. Increased expression at low glucose was maintained in these mutants. Hxt7 in the hxt null strain displayed an incomplete glucose repression. The growth rate correlated with the level of HXT7 expression at high glucose concentrations.