Two retrovirus vectors: pSIR-EGFP containing enhance green fluorescence protein (EGFP) reporter gene and pSIR-EGFP-870 with 870 bp p16 promoter were constructed on the base of self-inactivating retrovirus vector pSIR. Then retrovirus vectors were transducted into package cells for virus production and 2BS cells were infected with virus. During the course of reverse transcription, the 5′LTR lost its promoter activity.The transcriptional activity of p16 promoter in 2BS cells obviously increased during the aging process. The results demonstrate that self-inactivating retrovirus vector can be used for gene transcription regulation research during the aging process.