Microsatellite DNA Analysis of Somatic Cloned Goats
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This work was supported by a grant from The National Natural Sciences Foundation of China (39830280).

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    Abstract:

    Microsatellite DNA analysis was performed using ten pairs of goat microsatallite polymorphic DNA primers on two somatic cloned Jining grey goats, donor grey goat, recipient goats and three related control Jining grey goats. The result suggests that the amplification products are remarkably polymorphic in five pairs of goat microsatallite polymorphic DNA primers: SR-CRSP1, SR-CRSP5, SR-CRSP6, SR-CRSP7 and SR-CRSP24.The amplification products were silver stained after running on the 6% SDS-PAGE. The microsatellite DNA fingerprints of the two somatic cloned goats are the same as the donor, but are different from the recipient goat and all the control grey goats. It proves that the genomes of the two somatic cloned goats come from the donor cells.

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GUO Ze-Kun, GUO Ji-Tong, AN Zhi-Xing, ZHANG Yong, CHAI Yu-Bo, CHEN Nan-Chun, CHEN Su-Min. Microsatellite DNA Analysis of Somatic Cloned Goats[J]. Progress in Biochemistry and Biophysics,2002,29(4):655-658

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History
  • Received:December 28,2001
  • Revised:January 31,2002
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