Totally 236 unrelated individuals from majority population of Han Chinese in Beijing have been inspected with 9 polymorphism STR loci D3S1358,vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317,D7S820 by means of capillary electrophoresis and fluorescence multiple amplification. The results indicated a good correspondence with the Hardy-Weinberg equilibrium. Observed heterozygosity, expected heterozygosity,DP,PM,EPP,and PIC were calculated for evaluation of forensic application.