This work was supported by grants from The National Natural Sciences Foundation of China (30070267), Science Foundation for Postdoctors of China(2001), Science and Technology Foundation of Liaoning Province(2001226005).
The monoclonal antibody MABN1 against NMDAR could protect the neurons from excitotoxicity, but the mechanism is unknown. Cultured hippocampus neurons treated with glutamate or/and NMDAR antagonist (MABN1 and MK-801) were studied by FTIR spectroscopy. Spectroscopic differences were observed between MABN1-treated and MK-801-treated samples. Curve-fitting of the deconvoluted amideⅠband revealed the difference of protein second structure between MABN1-protected neurons and glutamate-treated neurons, by which it was presumed that MABN1 protects neurons against excitotoxicity by the mechanism different from the one of MK-801.
KANG Xiao-Nan, SUN Chang-Kai, FAN Ming, SONG Zhan-Jun, ZHAO Jie, WANG Ji-Qing, SHI Guang-Xia. Fourier Transform Infrared Spectroscopic Investigation of Excitotoxicity-protective Mechanism of Monoclonal Antibody Against NMDAR[J]. Progress in Biochemistry and Biophysics,2003,30(2):290-294
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