Centrosome is a tiny organelle which consists of two barrel-shaped centrioles surrounded by a fibrous meshwork termed as the pericentriolar material(PCM). The composition, morphology, size and position of the centrosome in a cell changes continually with cell cycle progression. Duplication of the centrosome is semiconservative and is coordinated with other cell cycle events, including DNA synthesis. Many centrosome-related proteins and kinases have been found to regulate different steps of centrosome duplication. Many growth-suppression genes such as p53, Rb, p21, Gadd45 and Brca1/2 are also involved in control of the duplication process. Centrosome abnormalities are associated with genomic instability and may play important roles in development of human cancers.