One novel tool and new methods used to punch the graft tissues and make the receptive blocks for constructing the tissue microarrays (TMAs) were devised. Two kinds of receptive blocks of TMAs consisting 390 and 448 spots of the graft tissues respectively were made successfully by the novel tool and new methods. These two kinds of TMAs sections had no loss of tissue spot. The H&E staining TMAs slides, which were observed under the light microscope, showed that the morphologic structure of all graft tissues had not be changed by punching, and the sections were appropriated thickness with symmetrical cells morphology. The positive expressions of P53 and P16 proteins in specimens of general sections were well consistent with that in TMAs sections by immunohistochemistry. The new tool and novel methods to punch the graft tissues and make the receptive blocks for constructing TMAs, which have many advantages such as low expense, easy operation, convenience and practicality, might be popular in future of the practical application in laboratory.