In order to identify whether EB virus latent membrane protein 1 (LMP1) can regulate vascular epithelial growth factor (VEGF) expression via STAT3 in nasopharyngeal carcinoma cell line, Western blotting was used to detect VEGF expression in HNE2, HNE2-LMP1 and HNE2-LMP1 transient transfected with STAT3 dominant negative plasmid STAT3β. VEGF is up-regulated by LMP1 and STAT3 dominant negative STAT3β can down-regulate VEGF expression. In tet-on-HNE2-LMP1 cell line, in which LMP1 can be tightly regulated, VEGF is expressed in dose and time dependent fashion. After cotransfected LMP1 with VEGF wild promoter luciferase reporter and 848 mutated luciferase reporter respectively, luciferase assay results indicated that LMP1 can up-regulate wild promoter luciferase activity and not that of 848 mutated luciferase reporter. Electrophoretic mobility shift assay results in HNE2 and HNE2-LMP1 cell line indicated that LMP1 enhances STAT3 binding ability in VEGF promoter. In conclusion, latent membrane protein 1 encoded by EB virus can enhance VEGF transcription and expression in nasopharyngeal carcinoma cell line via activating STAT3 binding ability in VEGF 848 promoter binding site.