An indirect enzyme-linked immunosorbent assay (ELISA) was developed to detect and quantitate the Klebsiella pneumoniae (Kp) capsule glycoprotein. The rabbit antiserum of Klebsiella pneumoniae capsule glycoprotein was prepared successfully by using a purified Kp capsule glycoprotein as antigen and the antibody was purified with ammonium sulfate fractionation and adsorption. The sensitivity of the assay is 0.031 mg/L. The standard curve is linear from 2.5 mg/L to 30 mg/L. The error of inter-plate is from 1.04% to 3.22%, and the error of intra-plate is from 2.61% to 8.35%.
LI Qing-Zhou, ZHANG Yan, QI Wei, DU Lian-Xiang. The Enzyme-linked Immunosorbent Assay of Klebsiella pneumoniae Capsule Glycoprotein[J]. Progress in Biochemistry and Biophysics,2004,31(6):567-571
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